Narrative:

I was vectoring aircraft #1 for sequencing for dca (to continue descent). Aircraft #2 was being vectored for climb (destination lga). They were separated until I climbed aircraft #2. When I saw the aircraft involved losing standard separation, I issued immediate turns to both aircraft away from each other. The 'conflict alert' did not activate until the error actually occurred.

Google
 

Original NASA ASRS Text

Title: ZDC CTLR ISSUES CLB CLRNC TO 2 ACR'S ON VECTOR WITH ENSURING LATERAL SEPARATION.

Narrative: I WAS VECTORING ACFT #1 FOR SEQUENCING FOR DCA (TO CONTINUE DSCNT). ACFT #2 WAS BEING VECTORED FOR CLB (DEST LGA). THEY WERE SEPARATED UNTIL I CLBED ACFT #2. WHEN I SAW THE ACFT INVOLVED LOSING STANDARD SEPARATION, I ISSUED IMMEDIATE TURNS TO BOTH ACFT AWAY FROM EACH OTHER. THE 'CONFLICT ALERT' DID NOT ACTIVATE UNTIL THE ERROR ACTUALLY OCCURRED.

Data retrieved from NASA's ASRS site as of July 2007 and automatically converted to unabbreviated mixed upper/lowercase text. This report is for informational purposes with no guarantee of accuracy. See NASA's ASRS site for official report.